Of the samples that were found, there were plenty of tintinnids from Newtown Creek and skeletonema in the Hudson. During our next days we hope to find more alga.
We are now focusing on the samples in the lab under an improve design. The focusing is on the affects of Nitrate and Ammonia enrichment. We have replicated the Hudson River’s environment to fit our experiment. Instead of only sampling the Hudson River we are also expanding to the East River, Newtown Creek.
During our last expedition we found that our set up was a success! Unfontany since our samples were moved and the floatation device was filled with water over time, the samples did not receive sunlight. Therefore, the samples were tarnished. We intend to improve our setup next week.
This time we tried two designs, that without the lobster trap and that with it. Both setups have our bottles, but we want to view how stable each environment may be from surrounding challenges .
Positives to both designs
Bag: 1. A great floating device
Lobster Trap: 1. Prevents outside sources from harming columns.
On Saturday, Professor Levandowsky and I ventured to the North Brooklyn Boating Club (NBBC) , to collect our samples by canoe. We found out that there was evidence of combined sewage overflow (CSO) in the East River when our test showed up for 0.3 ppm of ammonia.
When we got back to lab we found some warm and clam larva, but there was one thing that was stunning, we couldn’t figure it out.
Our collections from the Hudson River are still unrecovered. We are still concentrating on our set up. The biggest obstacle is keeping our samples in the water so that the experiment can occur. Some solutions that was thought of, were to keep our incubators in a lobster tap but that hasn’t worked . Instead, while our bottles were in the trap were punctured and water leaked in.
Our future studies revolves around our voyage to Newtown Creek in the East River. We intend to attempt our set up there as well.
As we dwindled down to the final days, Olive and I finally broadcasted our poster and oral presentation at the American Museum of Natural History. Here we, along with about 20 other teams are at the Urban Barcoding Research Program.
During our presentation we showed that in our results, that out of 20 samples 16 was successfully amplified. Of the 16 samples 10 were found as algal and six as non-algal.
Of our non-algal samples most blasts were found in the Animalia kingdom.
This is our second week and now Olive and I are going to finally see what two of our samples turn out to be. While we are here we are also labeling samples (in silica filled bags, and columns ) while referring them to the Master List I created.
We found out that our set up doesn’t work so we are going back to the drawing board. Solutions concludes to tying our samples with wire instead of line. We figured that the line untied over the week from the friction of the currents and the plank of wood – we had a bad week in New York.
We are going to finally set up our experiment, where we will create a weigh that will hold our samples down in the Hudson River as it’s tied to a plank with line that’s tied on the other end to a fence. As our samples stay afloat we hope to have them bloom.